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Identical control and test groups are used in both the C57Black and BALBC strains of mice. Mice are viagra 200mg noscript every two weeks for a total of three vaccinations. Blood samples are taken by orbital bleeds 2 weeks post-vaccination. Indirect ELISA assay is used to determine the titre of serum IgG specific to tetanus toxoid using an anti-mouse whole IgG antibody alkaline phosphatase conjugate (Sigma Cat No. A3562). Cell mediated immunity is measured by the delayed type lepsie ako viagra samples reaction. The right footpad of vaccinated or control mice are injected with 10 meg of tetanus toxoid in 0. 05 ml of saline and the left footpad is injected with saline only as a control.

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